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This liposome-forming compound is used for transfection of nucleic acids into a wide variety of eukaryotic cell types.A stable complex is formed when Escort IV is mixed with DNA in the absence of serum.There are many guides that help you select a transfection reagent.In general, consider: The type of cell(s) you will transfect The type of nucleic acid or protein you will introduce to the cell The composition of your cell culture medium The need for stable or transient transfection The equipment you have available The other factors important to you - cost, protocol flexibility, ease of use, etc.
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Cultured common cell lines are often immortalized, and generally continue to aquire mutations, leading to a heterogenous population that may perform differently from cells of lower passage number - leading to results that are not reproducible.